sh groups  (ATCC)

Journal: Stem Cell Research & Therapy

Article Title: HMGB1-mediated enhancement of glycolysis activates hepatic stellate cells by inhibiting ferroptosis in alcoholic hepatic fibrosis

doi: 10.1186/s13287-026-04970-1

Figure Lengend Snippet: HMGB1 regulated ferroptosis to affect HSC activation and ECM deposition. A IHC assay was used to observe the expression levels of GPX4 and FTH1 in liver tissues of AHF patients. B – E The levels of Fe 2+ /Fe 3+ , ROS, GSH content and MDA in LX-2 cells treated with alcohol at different concentrations were evaluated by kit detections. ( F – G ) qRT-PCR and WB assays were employed to assess the effect of elevated alcohol concentration on the mRNA and protein levels of NRF2, GPX4, FTH1, SLC7A11 in alcohol-stimulated LX-2 cells. H – J CCK-8, EdU staining and IF assays were respectively utilized to detect the effect of Erastin on the viability, proliferation, activation and ECM deposition of alcohol-induced LX-2 cells. K The effect of Erastin on lipid peroxidation was assessed by flow cytometry using the C11-BODIPY 581/591 probe in ethanol-induced LX-2 cells. L – P The effects of Ferrostatin 1 on si-HMGB1 regulating the levels of lipid peroxidation, Fe 2+ /Fe 3+ , ROS, GSH content and MDA in alcohol-treated LX-2 cells were measured by kit detections. Q – R qRT-PCR and WB assays were used to evaluate the effects of Ferrostatin 1 on si-HMGB1 regulating the mRNA and protein levels of NRF2, GPX4, FTH1 and SLC7A11 in alcohol-stimulated LX-2 cells. S – T The effect of Ferrostatin 1 on si-HMGB1 regulating the expression of GPX4, FTH1 and HMGB1 in LX-2 cells treated with alcohol was observed by double-labeled IF assay. * p ˂ 0.05, ** p ˂ 0.01, *** p ˂ 0.001 vs. 0 µg/mL Ethanol/ Ethanol/ Ethanol + si-NC/ Ethanol + si-HMGB1

Article Snippet: Mice in the Model + sh-HMGB1 + Ferrostatin-1 group received an intraperitoneal injection of Ferrostatin-1 (1 mg/kg/d) (HY-100579, MedChemExpress, New Jersey, USA) from weeks 6 to 8, while otherwise following the same modeling protocol as described above.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Concentration Assay, CCK-8 Assay, Staining, Flow Cytometry, Labeling

Journal: Stem Cell Research & Therapy

Article Title: HMGB1-mediated enhancement of glycolysis activates hepatic stellate cells by inhibiting ferroptosis in alcoholic hepatic fibrosis

doi: 10.1186/s13287-026-04970-1

Figure Lengend Snippet: sh-Hmgb1 modulated glycolytic metabolism, ferroptosis, and liver fibrosis in AHF mice. A – B H&E staining and Masson staining were used to observe the effect of sh-Hmgb1 on hepatic fibrosis in AHF mice. C – D The effects of sh-Hmgb1 on Hmgb1‌ mRNA and protein levels in liver tissue of AHF mice were assessed by qRT-PCR and WB assays. E – H Kit and IHC assays were utilized to evaluate the effect of sh-Hmgb1 on the markers related to glycolysis, ferroptosis and HSC activation. * p ˂ 0.05, ** p ˂ 0.01, *** p ˂ 0.001 vs. Normal/ Model + AAV-sh-NC

Article Snippet: Mice in the Model + sh-HMGB1 + Ferrostatin-1 group received an intraperitoneal injection of Ferrostatin-1 (1 mg/kg/d) (HY-100579, MedChemExpress, New Jersey, USA) from weeks 6 to 8, while otherwise following the same modeling protocol as described above.

Techniques: Staining, Quantitative RT-PCR, Activation Assay

Journal: Stem Cell Research & Therapy

Article Title: HMGB1-mediated enhancement of glycolysis activates hepatic stellate cells by inhibiting ferroptosis in alcoholic hepatic fibrosis

doi: 10.1186/s13287-026-04970-1

Figure Lengend Snippet: Hmgb1 influenced liver fibrosis in AHF mice by regulating ferroptosis. A – B H&E staining and Masson staining were utilized to observe the effect of Ferrostatin-1 on sh-Hmgb1 mediating liver fibrosis in AHF mice. C – E The effects of Ferrostatin-1 on sh-Hmgb1 regulating the levels of ROS, GSH content and MDA in liver tissues of AHF mice were assessed by kit detections. F – G IHC assay was employed to measure the effect of Ferrostatin-1 on sh-Hmgb1 regulating the markers related to ferroptosis and HSC activation. * p ˂ 0.05, ** p ˂ 0.01, *** p ˂ 0.001 vs. Model + AAV-sh-NC/ Model + AAV-sh-Hmgb1

Article Snippet: Mice in the Model + sh-HMGB1 + Ferrostatin-1 group received an intraperitoneal injection of Ferrostatin-1 (1 mg/kg/d) (HY-100579, MedChemExpress, New Jersey, USA) from weeks 6 to 8, while otherwise following the same modeling protocol as described above.

Techniques: Staining, Activation Assay